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Whole Exome Sequencing (WES)
- Tumor Only or
- Tumor/Normal Paired analysis
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Hybrid Capture Technology
- Customized KAPA Biosystems Hyper Prep Automated library preparation (Perkin Elmer Sciclone)
- Agilent SureSelect Capture Baits (V6+COSMIC)
- Illumina NovaSeq 6000 sequencing platform
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Tumor Only:
- SNV and Indels calling
- Copy Number
- Microsatellite Instability (MSI)
- Loss of Heterozygosity (LOH); HLA typing
- Mutational Signature; All mutation burden
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Tumor/Normal Paired:
- SNV and Indels calling
- Somatic copy number; Ploidy estimation; Cellularity
- Microsatellite Instability (MSI)
- Loss of Heterozygosity (LOH); HLA typing
- Neoantigen prediction
- Mutational Signature; Somatic mutation burden
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RNA Sequencing (RNASeq)
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Transcript-based Probe Capture Technology
- Illumina TruSeq RNA Exome Automated library preparation (Perkin Elmer Sciclone)
- Illumina NovaSeq 6000 sequencing platform
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- Gene expression analysis
- RNA variant calling
- Gene fusion calling, including intragenic fusion events
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ctDNA Sequencing
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Hybrid Capture Technology with unique molecular indices (UMIs) and error correction algorithms
- Illumina TruSight Oncology 500 cfDNA (TSO500) library preparation
- Illumina NovaSeq 6000 sequencing platform
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- SNV and Indels calling
- Clinically relevant fusions
- Copy Number Variations (CNVs)
- Tumor Mutational Burden (TMB)
- Microsatellite Instability (MSI)
- Explore mechanisms of response and resistance without the need for matched baseline and on-study tumor biopsies
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Targeted NGS assay (DNA and RNA)
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Targeted Amplicon Gene Panel Technology
- Oncomine Comprehensive Assay (OCAv3)
- Detects more than 8000 variants in 161 cancer-related genes across all major cancer types
- Ion AmpliSeq library preparation
- Ion Torrent Ion S5 sequencing platform
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- SNV and Indels calling
- Gene fusion calling
- Copy Number Variations
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